Theoretical and Experimental Approach Towards P-Cyano Stilbene Schiff Base as a Potential Linker in E-DNA Sensor

摘要

This research aim to synthesis and characterize several p-cyano stilbene Schiff base molecules as a potential linker in electrochemical DNA sensor. Schiff base reaction is form from condensation reaction between primary amine and aldehyde at 79 °C using ethanol as solvent. All compounds are investigated and discussed by Fourier transform-infrared spectrometer (FTIR) and Uv-vis spectrophotometer. For computational study, structure drawings were constructed using Gauss View 5.0. Geometry optimization has been carried out using density functional theory (DFT) at bB3LYP/6 31G and performed using Gaussian09 software. FTIR showed formation of C=N stretching vibrations at 2224 cm~(-1) also another important stretching at 1604 cm~(-1) and 1249 cm~(-1) which represent C=N and C-O mode respectively. In UV-vis, absorbance of C=N (imine) group can be observed at peak range 363 nm and transition of aromatic C=C can be seen at 238 nm. Theoretical and experimental results obtained are comparable and p-cyano stilbene Schiff base compounds were successfully synthesised and can be further applied as a linker in E-DNA sensor.