Absorbance Analysis of Escherichia coli (E. coli) Bacteria Suspension in Polydimethylsiloxane (PDMS)-Glass Based Microfluidic

摘要

The emerging bacteria/cell culturing in biological/biomedical research and industry is in demand as a low cost, fast, non-invasive, and reliable alternative/approach for evaluation and measurement. Microfluidic approach is one of the promising alternatives for replacing the expensive commercial cuvette for bacteria/cell culture and suspension for optical measurement. This study demonstrates the integration of absorbance measurement with microfluidic platform for Escherichia coli (E. coli) bacteria suspension analysis. The E. coli was cultured in suspension medium and was transferred inside the PDMS-glass based microfluidic. The absorbance measurement was carried out using UV-Visible spectrophotometer. We had demonstrated this method by measuring absorption of light transmitted through microfluidic chambers within the visible light range of 350 nm to 750 nm. From the result, it indicated that the graph pattern and growth behavior of E. coli suspension in microfluidic platform are reliable and comparable to commercial Quartz cuvette reading.