In vitro evaluation of serological cross-reactivity and cross-neutralization between the US PEDV prototype and variant strains

摘要

Porcine epidemic diarrhea virus (PEDV) was detected in US swine for the first time in April 2013.' Initially it was thought that there was only one PEDV strain circulating in US swine (designated as US PEDV prototype strain).2 However, in late January2014, our group identified a distinct PEDV strain in US swine (designated as US PEDV variant strain) that is genetically different from the US prototype strain. Little is known about the phenotypic features of this US PEDV variant. The current immunofluorescence antibody (IEA) assays, enzyme-linked immunosorbent assays (ELISA) and virus neutralization (VN) tests offered at veterinary diagnostic laboratories for detection of PEDV-specific antibody are based on the US PEDV prototype strain. It remains to be determined if these assays based on the US PEDV prototype strain can detect antibodies against the US PEDV variant strain and vice versa.