3 3 Variant ORF3 protein derived from PEDV a nuceleic acid encoding the protein method of differentiating wild type and attenuated PEDV using the variant ORF3 protein and gene thereof and a kit for differentiating wild type and attenuated PEDV

摘要

A porcine epidemic diarrhea virus(PEDV) ORF3 polypeptide which includes 17 amino acids deletion compared to a wild type is provided to play an important role in attenuating the PEDV. A method for detecting attenuated PEDV and a kit for detecting the attenuated PEDV are provided to distinguish a wild type PEDV from an attenuated PEDV easily. A PEDV ORF3 polypeptide includes an amino acid sequence described as SEQ ID : NO. 1. A method for detecting attenuated PEDV comprises the steps of: (a) amplifying an ORF3 gene from a sample including PEDV using a primer set including a first primer binding to the upstream of a nucleotide at position 245 of the ORF3 gene and a second primer binding to the downstream of a nucleotide at position 295 of the ORF3 gene; and (b) identifying the existence of the amplified product or analyzing the size of the amplified product. A kit comprises the primer set and operation instructions.