Layer-by-layer Assembled Enzyme Cascade Bioanode for Catalyzing Oxidation of Sucrose

摘要

We report here a novel layer-by-layer (LbL) assembled enzyme cascade bioanode incorporated with CNT-invertase and CNT-glucose dehydrogenase for sucrose oxidation in a biofuel cell. Methylene green (MG) was used as redox mediator for electrocatalytic oxidation of NADH at reduced overpotentials on screen printed electrode (SPE). The LbL architecture showed advantages for sequential enzymatic reaction that favored the efficient penetration of substrate and products in a cascade system. The maximum current density and power density achieved were 412 ± 36 μA/cm~2 and 85±6 μW/cm~2.The high current density/power density obtained showed LbL assembly is of great advantage and can be employed as a simple and efficient way to construct enzyme cascade bioanode for biofuel cell applications.