Stabilization and Controlled Release of Invertase Through the Addition of Trehalose in Wet and Dried Alginate-Chitosan Beads

摘要

1. Dried bead microstructure depended on both drying method and composition, leading to lower enzyme release rates from alginate-trehalose freeze-dried beads, which showed smooth surface with no porous structure. 2. The use of a semiempirical equation allowed the establishment of certain characteristics of the enzyme release mechanism. Trehalose and chitosan inclusion, hydration degree, and drying method greatly influence the transport mechanism of enzyme release in alginate beads. 3. The combination of encapsulation in alginate-chitosan beads with drying immobilization in an amorphous trehalose matrix, which remained in a glassy state at ambient temperature, was an efficient strategy to achieve enzyme stability and its controlled release.