Development of a Method for Producing and Purifying N2-Carboxyethyl-2'-Deoxyguanosine for Molecular-Biological Research

摘要

Methylglyoxal (MG) – is a highly reactive α-oxoaldehyde, which can be synthesized in vivo through several biochemical pathways. MG reacts with nucleophilic groups of proteins, lipids and nucleic acids forming advanced glycation end products. Advanced glycation end products play an important role in the pathogenesis of diabetes, chronic inflammation, cancer and Alzheimer's disease. One of the most stable compounds, which is formed by reacting of methylglyoxal with the nitrogenous bases of the DNA molecule is N~2-carboxyethyl-2'-deoxyguanosine (CEdG). This compound coudbe used as a marker for monitoring of various diseases, however its applicability is restricted by high cost of the analytical standards. To perform our molecular-biological investigations by mass spectrometry, we synthesized N~2-carboxyethyl-2'-deoxyguanosine, and have proposed a new, cost efficient method for purifying compounds by reverse - phase HPLC. As a result, we achieved the optimal purity of the sample, while substantially reducing the cost of the purification procedure.