A study was carried out to test the cytotoxicity and antiviral effects of methanolic extracts from the leaves and stem of Polygonum minus or kesum. Cytotoxicity tests were performed on Vero cells indicates the LC_(50) value for leaf extract towards the Vero cells was 875 mg/L and the LC_(50) value for stem extract was 95 mg/L. The LC_(50) values indidcate the non-cytotoxic effect of the extracts and worth for further testing. Antiviral test were carried out towards herpes simplex virus infected Vero cells using three concentration of extract which were equivalent to 1.0 LC_(50), 0.1 LC_(50) and 0.01 LC_(50). Three different treatments to detect antiviral activity were used. Mild antiviral activity of the stem extract was detected when cells were treated for 24 hours with plant extract before viral infection. This demonstrates the capability of the test compound to protect the cells from viral attachment and of the possible prophylactic effect of the P. minus stem methanol extract.
展开▼
机译：进行了研究以测试甲醇溶液从叶片和叶片的甲醇萃取物的细胞毒性和抗病毒作用。在Vero细胞上进行细胞毒性试验表明叶片提取物的LC_（50）值朝向VERO细胞为875mg / L，STC萃取物的LC_（50）值为95mg / L. LC_（50）值偶达于提取物的非细胞毒性效果，值得进一步测试。使用三种浓度的提取物对疱疹病毒感染的Vero细胞进行抗病毒测试，其等同于1.0LC_（50），0.1LC_（50）和0.01LC_（50）。使用三种不同的治疗方法来检测抗病毒活性。当在病毒感染前用植物提取物处理细胞24小时时，检测到干提取物的温和抗病毒活性。这证明了试验化合物保护细胞免受病毒附着的能力和P.P.P3的可能预防作用。
展开▼
