Determination of magnolol and honokiol in Mangnolia officinalis by Near-infrared Spectroscopy Rapidly

摘要

In this paper, Near-infrared spectrum technology was applied for fast determining the content of magnolol and honokiol in Mangnolia officinalis. 105 samples of Mangnolia officinalis from xx pharmaceutical factories were divided into a calibration set (79 samples) and a validation set (26 samples). The near infrared spectrum of samples were collected, while take the HPLC value as a reference, Use the partial least squares (PLS) to establish the quantitative model of Magnolol and Honokiol. The correlation coefficient of calibration (R ), the root-mean-square error of calibration(RMSEC) and the root-mean-squareerror of cross-validation(RMSECV) of the calibration model were 0.9813, 0.0833, 0.2518; the recovery rate of prediction of the validation was 99.99%.The method is uncomplicated and result is accurate, which could measure the content of Mangnolia officinalis in bulk and fast, save costs and improve efficiency.