The Anti-Photoaging Efficacies of Ganoderma lucidum Extracts

摘要

Ganoderma lucidum (GL) was listed in ShenNong's Classic of MateriaMedica as a superior medicine,which provides the effect on maintaining health without toxicity even for long-term use,as well as benefits for energy and life-enhancement.Thepurposes of this study were to isolate different molecular weights (MW) of GLpolysaccharides,examine the antioxidant efficacy,and analyze the anti-photoaging effects.Furthermore,the polysaccharides of GL presented non-cytotoxic to mouse skin fibroblasts (NIH3T3),and the survival rates of 0.5mg/mL of GL polysaccharides treatments for 48 hours are more than 102％.Finally,the results of anti-photoaging assays showed that llμg/ml of G.lucidum polysaccharides presented the respective abilities to protect NIH3T3 cell lines to against 25 mJ/crn2 of UVB treatments.The cell viabilities of G.lucidum polysaccharides treatments were 98.89％ (above 20 kDa MW) and 108.15％ (below 20 kDa MW),which were much higher than the control group (59.32％).These indicated that G.lucidum polysaccharides were able to protect cells from UVB damage effectively.G.lucidum polysaccharides (1 μg/ml) could repair UVB damaged cells,and further increased cell survival up to 99.05％ (above 20 kDa MW) and 101.8％ (below 20 kDa MW).In conclusion,G.lucidum has high potential to develop the anti-photoaging skin care products.