Determination of Bacillus Calmette-Guerin Concentration Integrated in Delivery Materials for Intravesical Infusion Therapy of Superficial Bladder Cancer

摘要

Purpose: To assess determination of Bacillus Calmette-Guerin(BCG) concentration integrated in delivery materials for intravesical infusion therapy of superficial bladder cancer. Material and Methods: The standard BCG solution was prepared and divided into two groups. One group was measured by spectrophotometer direct, the other group was determined by using enzyme mark instrument after the solution had cultured. The preliminary experiment study on BCG integrated delivery materials was finished by XTT method. Results: The wavelength of the characteristic peak changes with the variety of the concentration measured by spectrophotometer direct. The concentration of BCG which was embeded in integrated delivery materials for intravesical infusion therapy of superficial bladder cancer was linear with absorbance at 450nm by XTT method in the selected range.