Plasmids role in survival of Acinetobacter calcoaceticus A1 exposed to UV-radiation and hydrocarbons

摘要

The role of plasmids in hydrocarbon-degrading bacteria Acinetobacter calcoaceticus Al survival to UV-radiation and hydrocarbons was studied. Natural plasmids-containing A. calcoaceticus Al showed high resistance to UV-radiation. A. calcoaceticus Al showed active growth under exposed to UV- radiation for up to 30 minutes. Combined effects of UV-radiation and petroleum hydrocarbons did not considerably reduce the growth of strains. It was shown a stimulating effect of UV-radiation on the growth curves of strains of A. calcoaceticus Al. Constructed recombinant strain (E.coli XL blue Rec) showed the ability to grow on medium with addition petroleum hydrocarbons. Combined effects of UV- radiation and petroleum hydrocarbons have had a negative effect on the growth of E.coli XL blue Rec. Thus, results showed that the plasmid DNA of natural hydrocarbon-degrading bacteria A. calcoaceticus Al may contain genes of microbial resistance to UV - radiation and petroleum hydrocarbons.