Association between Genetic Polymorphisms of 5, 10-Methylenetetrahydrofolate Reductase and Cytokinesis-Block Micronucleus in Peripheral Blood Lymphocyte among 1,3-Butadiene Workers

摘要

Exploring the associations between genetic polymorphisms and susceptibility of chromosomal damage induced by 1,3-butadiene(BD) is of much importance for understanding BD carcinogenesis. In current study, we investigated the effects of MTHFR polymorphisms and MTHFR gene haplotype pairs on chromosome damage detected by the cytokinesis-block micronucleus (CBMN) assay in peripheral blood lymphocytes. The data showed that the frequencies of CBMN among BD-exposed workers were significantly associated with the MTHFR A1298C polymorphism (P=0.04 and P=0.03, respectively) by multivariate analysis of covariance. The foundings also showed that the haplotype pairs in the MTHFR gene were more likely than single SNPs to correlate with the BD-induced chromosome damage among occupational exposed workers. Gene-environment interactions between occupational BD exposure and polymorphisms of MTHFR were also evident. These results indicate that the MTHFR polymorphisms may play a role in sensitivity or genetic susceptibility in genotoxic effects of BD exposure in the occupational exposure population.