An aptamer-nanogold probe for trace PDGF using resonance Rayleigh scattering as detection technique

摘要

The aptamer of platelet-derived growth factor (PDGF) was used to modified nanogold to obtain a stable aptamer-nanogold(Apt-NG) probe for PDGF. In the condition of pH 7.2 Na_2HPO_4-NaH_2PO_4 buffer solution and 5.33 mmol/L NaCl, the Apt-NG specifically combined with PDGF to form Apt-NG-PDGFA cluster that resulted in the resonance Rayleigh scattering (RRS) intensity at 550 nm. When the PDGF concentration increased, the nanogold aggregated (NGA) increased, and the RRS intensity at 550 nm increased. The increased RRS intensity ΔI_(550nm) was linear to the PDGF concentration in the range of 3.33-40 ng/mL, with a regression equation of ΔI_(550nm)=10.8 C-24.2, coefficient of 0.9932 and a detection limit of 0.53 ng/mL.