Using Loop-mediated Isothermal DNA Amplification (LAMP) and Spectral Surface Plasmon Resonance (SPR) to Detect Methicillin-resistance S. aureus (MRSA)

摘要

Loop-mediated isothermal amplification (LAMP) is a rapid (within an hour) and sensitive method to amplify DNA at one temperature. It has a high potential to be used as a biosensor to detect pathogens as LAMP does not require thermal cycles to amplify DNA. On the other hand, surface plasmon resonance (SPR) is a highly sensitive optical system for label-free biomolecules detection. With these advantages, we tried to combine SPR and LAMP to detect the gene of Panton-Valentine leukocidin toxin (pvl) of Methicillin-resistance S. aureus (MRSA). Our preliminary data indicate that the LAMP amplification products (lamplicons) of pvl showed an obvious spectrum shift when monitored by SPR. Our data suggest that using SPR to sense the LAMP products is a new approach for real-time biosensing.