Purification and characterization of a yam glycoprotein isolated by alkaline extraction from yam tuber

摘要

Yam glycoprotein (YGP) is an important source of bioactives for functional foods. To investigate the effects of alkaline extracting method on the features of YGP, A glycoprotein was isolated from yam tubers by using alkaline processing method, and purified by ion-exchange and gel filtration column chromatography. During the SDS-PAGE eletrophoresis, the result shows a band with approximately 30 kDa molecular weight. The YGP consists of protein moiety (62.34%) and carbohydrate moiety (37.51%), respectively. By the automatic amino acid analyzer detecting, it indicated that the YGP consists of 17 kinds of amino acids and contains a high percentage of glutamic acid and aspartic acid. The results of thin-layer chromatography show that oligosaccharides of the YGP contain D-glucose, D-galactose and mannose.