The comparison of physicochemical properties of four Cross-linked sodium hyaluronate gels with different cross-linking agents

摘要

Purpose The physicochemical properties of four cross-linked sodium hyaluronate gels (CHA) with different cross-linking agents were compared in order to research out the different stability and Enzyme-resistant degradation properties of these CHA gels. Methods The CHA hydrogels were prepared with different cross-linking agents, such as PEG20000, PDE, BDDE and ADH. The optimal reaction conditions were determined by single factor experiment. Dynamic viscosity was tested by Stabinger method. Intrinsic viscosity was determined by Uzziah's viscosity method. The enzyme-resistant degradation properties in vitro of CHA-gels were analysed by carbazole and spectrophotometry. Results the concentrations of NaOH/HCl, concentrations of HA and the ratio of cross-linking agent to HA are major factors of conditions which influenced the physicochemical properties of CHA gels. PDE-CHA and PEG20000-CHA gels possess better Dynamic viscosity, PDE-CHA gel has also better intrinsic viscosity, ADH-CHA and BDDE-CHA gels get better Enzyme-resistant degradation properties than PEG20000-CHA and PDE-CHA gels. Conclusion The CHA-gels prepared under optimal reaction conditions have different physical and chemical properties, which set foundation for developing double cross-linked or multifunctional gels with both excellent stability and enzyme-resistant degradation properties.