Surface anchored poly(methylhydrosiloxane) (PMHS) films (≈10 nm - 1μm) were functionalized with 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. The interface was very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, cytochrome C and α-chymotrypsin at pH 7.4 as observed by quantitative normal scanning confocal fluorescence. The surface is also repellent to liposomes (400 nm diameter) of L-α-phosphatidylcholine (Soy-20%). Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups.
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