Bioconversion of Primary Sludge to Fermentable Sugars for Biofuel Applications(PPT)

摘要

1. PS had a high carbohydrate content (62.3%) and low lignin content (8.1%) which is considered a good feedstock for biorefinery applications 2. Treatment of PSS with HC1 removed 64% of ash 3. PS deashing at 10% solids improved glucose recovery 21-fold over unwashed control 4. Glucose recovery in presence of non-ionic surfactant (PEG4000) increased more than 2-fold over deashed control 5. Max glucose yield of approx. 70% was obtained at: 9.6 wt% solids, 1.8 wt% enzyme in presence of 1 wt% w/w PEG 4000 after 91 h hydrolysis time 6. Enzyme recovery of 37% of total enzyme was achieved using UF method (3 kDa) 7. Recovered enzyme supplemented with new enzyme (1:3 ratio) performed as good as fresh enzyme (70% glucose yield) 8. Ethanol yield of 0.47 g/g glucose represents 92% of the theoretical maximum 9. Growth independent lipid production with a max lipid content of 13.6 wt% (further optimization required) 10. Good hydrolysate fermentability indicative of low inhibitory levels 11. PS - promising feedstock for cost-effective and sustainable production of biofuels and biochemicals.