Fabrication of Silver Nanoparticles Immobilzed Microfluidic Chip for Chemiluminescence based Analytical Application

摘要

Silver nanoparticles (Ag NPs) were successfully immobilized on polydimethylsiloxane (PDMS) surface in the microfluidic chip through surface modification using 3-aminopropyletriethoxy silane (APTES) followed by plasma treatment. The presence of Ag NPs was confirmed by UV-Vis and SEM-EDS spectra. The fabricated microfluidic chip was applied to determine the concentration of L-Cysteine (Cys) sensitively by luminol-H_2O_2 chemiluminescence (CL) system through its inhibition effect. The linearity of the CL signal from Luminol-Cys-H_2O_2 system in the fabricated sensor chip was observed in the range of 1.2 to 10.5 μM (r=0.9983) of Cys with a limit of detection (LOD) of 33 nM.