Silver nanoparticle-initiated chemiluminescence reaction of luminol-AgNO_3 and its analytical application

摘要

Ag~+ has been regarded as an inert chemiluminescent oxidant. In this work, it was found that in the presence of silver nanoparticles (AgNPs), AgNO_3 could react with luminol to produce strong chemiluminescence (CL). The AgNPs with smaller size could initiate stronger CL emission. To investigate the CL mechanism of the AgNPs-luminol-AgNO_3 system, the UV-visible spectra and the CL spectrum of the CL system were obtained. The CL reaction mechanism involving catalysis was proposed. Compared with the reported nanoparticles-luminol-H_2O_2 CL system, the AgNPs-luminol-AgNO_3 CL system has the advantages of low background and good stability. Moreover, the new CL system was used in immunoassay for IgG. [Figure not available: see fulltext.]