Multiplexed Electrochemical Immunosensor for Detection of Celiac Disease Serological Markers

摘要

Celiac disease is a gluten-induced autoimmune enteropathy characterized by the presence of antibodies against gliadin (AGA) and anti-tissue transglutaminase (anti-tTG) autoantibodies. A disposable electrochemical dual immunosensor for the simultaneous detection of IgA and IgG type AGA and anti-tTG antibodies in real patient's samples is presented. A screen-printed carbon electrode with two working electrodes nanostructured with a carbon-metal hybrid system worked as the transducer surface. The immunosensing strategy consisted of the immobilization of gliadin and tTG (i.e. CD specific antigens) on the nanostructured electrode surface followed by the electrochemical detection of the human antibodies present in the serum samples assayed. The antigen-antibody interaction was recorded using alkaline phosphatase labelled anti-human antibodies and a mixture of 3-indoxyl phosphate with silver ions (3-IP/Ag+) was used as the substrate. The analytical signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with a commercial ELISA kit indicating that the electrochemical immunosensor is a trustful analytical screening tool.