IrO_x and Pt-Ir Electrochemical Sensors: Prospective Enzyme-less pH and Glucose Sensors for Continuous Monitoring in Cell Culture

摘要

This study reports the development of two enzyme-less biosensors by using IrO_x for pH and Pt-Ir for glucose sensors. Nanostructures of electrodeposited IrOx and Pt-Ir were examined by SEM and XPS on the electrode surfaces. We used chronopotentiometry (CP) for pH and linear sweep voltammetry (LSV) for glucose measurement at 37°C in 1X PBS (0.15M NaCl). In the physiological range of pH 4-10 and glucose concentration 1 mM-12 mM, these two biosensors exhibited good linearity of -68.5 mV/pH (R~2:0.99) and, 0.97 mA/mM-dm~2 (R~2:0.988), respectively. Because the linear ranges are suitable for cell culture, the presented electrochemical sensors are prospective for applying to continuous cell culture monitoring.