Monoclonal Antibody Binding to (Fluoro) Quinolones in Competitive Immunoassay: Quantitive Correlation Antibody Affinity with Molecular Parameters

摘要

@@ Antibodies used in competitive immunoassay tor the detection ot small molecule usually recognize one more molecules that share common epitopes with different affinities. For immunochemist, there is a challenging problem to well understand the cause of the antibody' s multi-specificity from only looking two-dimensional structural formulas of the compounds studied (Sanvicens et al.，2003). A method that can provide useful parameters about the topological and electrostatic properties of molecule can be sufficient in elucidating the diversity of antibody binding (Beier and Stanker 2001). In the study, we determinates the affinities of one monoclonal antibody (C4A9H1) to 14 (fluoro) quinolones by competitive ELISA and calculated molecular parameters of these (fluoro)quinolones by quantum chemical B3LYP/6-31G*.