T-RFLP analysis of soil microbia! community from Shandong Province for forensic science

摘要

Soil microbial community very little had been found as trace evidence, however, itcould be vital for forensic science, In this paper, soil microbial community diversities from seventeen different regions of Shandong province were investigated by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The results of diversity index analysis show the highest Margalef index and Eveness index are 4.609 for sample D and 0.970 for sample E, respectively. In T-RFLP profile, the absolute advantage peak are 488bp (62.6% ) from sample O and 496.5bp (20.8%) from Sample H. The analysed terminal restriction fragments (T-RFs) are from Firmicutes and Proteobacteria. These results reveal that there are the obvious regional characteristic for microbial community of all the detected samples, which suggests T-RFLP analysis of microbial community diversity will be a rapid effective fingerprinting method for forensic detection.