Research on determination of clenbuterol and salbutamol in pork by SPE-HPLC

摘要

A method determining the clenbuterol and salbutamol in pork was developed. The method was based on SPE(C18),separated by ZOBAX SB C18,and using a mobile phase of 0.01mol/L Sodium dihydrogen phosphate - Acetonitrile in gradient clution, detected with wavelength 210nm. The linear relations in the scope of 1.0～10mg/kg, 3.0～30mg/kg. The recoveries ranged from 91.3%～97.2%, 79.7%～89.3%. The detection limits 0.02mg/kg, 0.05mg/kg.