Structure and antibacterial activity of silk fibroin/chitosan nanofibrous mats using an electrospinning technique

摘要

More and more attention has been paid to natural materials. This paper was carried out with an intention to investigate the effect of electrospun silk fibroin (SF) nanofibrous mats by mixing different weight raito of chitosan (CS) in the same solvent-formic acid. Based on good electrospining feasibility, it was found that the average diameter of spun fibers became fine from 337nm to 103nm, with the concentration of CS in the blend compositions increasing to 4%. At the same time, the conformational transition of SF nanofibers adding CS occured to the tendence towards β-sheet structure by means of the analysis of the Fourier transform infrared spectroscopy (FTIR), Wide angle X-ray diffraction (WAXD) and Differential thermal analysis (TGA). What was more, excellent antibacterial activity (a degree growth inhibition of more than 95%) of the promising nanofibrous mats was revealed, through utilizing the colony counting method against Gram-positive bacteria S. aureus and Gram-negative bacteria E.coli, which would open up wide applications on would dressing, filtration and environmental purification.