Observations on the antimicrobial susceptibility of Staphylococcus pseudintermedius following the introduction of cefovecin for clinical use in Europe

摘要

In vitro susceptibility tests were conducted on field isolates of Staphylococcus pseudintermedius from canine clinical cases isolated from European countries during 2009-2010 for sensitivity to cefovecin and a panel of other commonly used antimicrobials by broth microdilution and oxacillin disc diffusion tests. Isolates suspected of multi-drug resistance were screened for the presence of the mecA gene. Eight isolates with zone diameters of less than 16 mm in the oxacillin disc diffusion test were deemed resistant to oxacillin and, following genotyping, seven were confirmed mecA positive. The MIC_(90) and MIC_(50) values for cefovecin were both 0.25 μg/mL and those isolates selected for genotyping had MIC values for cefovecin of ≥1 μg/mL. The results for cefovecin were compared with data from a similar survey conducted prior to launch in 1999-2003. The data indicate that to date for canine clinical isolates of S. pseudintermedius, there has been no apparent shift in susceptibility to cefovecin amongst isolates from first opinion cases across Europe but continued monitoring is recommended.