Effect of Chlorogenic acid on H_2O_2-induced dysfunction and apoptosis in HUVECs

摘要

To investigate the protective effects and the possible molecular mechanisms of chlorogenic acid on H_2O_2-induced dysfunction and apoptosis in human umbilical vein endothelial cells (HUVECs). HUVECs were divided into three groups: H_2O_2 groups (0, 5, 10, 20, 50mM), chlorogenic acid (100nmol/L) groups and atorvastatin (10μmol/L) groups. The eNOS mRNA expression was measured by RT-PCR. The content of NO in the supernatant was detected by the nitrate reductase assay. Cells activity was evaluated by the MTT assay. The level of reactive oxygen species (ROS) in cells was measured using H_2DCF-DA as a fluorescence probe. Results demonstrated that H_2O_2 caused a significant down-regulation of eNOS mRNA expression and decreased the secretion of NO in a dose-dependent manner compared with the control group. Furthermore, H_2O_2 induced cell apoptosis, increased ROS accumulation. Nevertheless, these effects of H_2O_2 could be blocked by chlorogenic acid in chlorogenic acid pretreatment groups. CONCLUSION:chlorogenic acid can inhibite H_2O_2-induced dysfunction and apoptosis in HUVECs, which may be related to its effects on suppressing oxidative stress, up-regulating eNOS pathway. These results suggest the usefulness of chlorogenic acid particularly in the treatment of cardiovascular disease.