Phylogenetic status of dominant species H-15 in Chenxiang Tea of Guangdong during piled up fermentation

摘要

Its important for revealing the formation mechanism of quality and flavor of Chenxiang Tea of Guangdong that confirm the taxonomic status of dominant fungi species in the tea during its piled up fermentation. A dominant fungus H-15 was isolated from the Chenxiang Tea during its piled up fermentation, and classification had been conducted by morphological and molecular biology methods. 5.8SrDNA-ITS sequence of H-15 was amplified by universal primers, and phylogenetic tree was conducted to analysis its classification status, results shows that H-15 could be classified as black group of Aspergillus with closest relationship with A.niger, A. tubingensis and A. foetidus. Results of classical classification methods studies showed that H-15 also be classified as black group of Aspergillus, by colony morphology and the optical microscope to observe the structure of its sporulation, but all of these results couldn't enough to confirm the taxonomic status of H-15 to species level. However, ultrastructure of its spores was observed by electron microscopy, and the spore surface was roughness, thrusting, spike, ridges, occasional smooth surface, all of these characteristics of spores of Aspergillus is identity with those of A.niger.