Rapid Determination of Formaldehyde in Dried Bean Milk Cream by HPLC with Pre-Column Derivatization

摘要

A high-performance liquid chromatography method was set up for rapid determination of formaldehyde in dried bean milk cream. This thesis studies the Nash derivatization, derivative solubility, reaction time, amounts of Nash and derivative stability. The derivative is chromatographic separated by Agilent Zorbax Eclipse XDB-C18 column (4.6 mm×250 mm, 5μm) and detected by index detector with VWD (412nm). The heater does not need the temperature-controlling system. A mobile phase was composed of acetonitrile and water (50:50, V/V) at a flow rate of 0.8 mL/min. Under the conditions of the above-mentioned test, the developed calibration curves displayed good linearity over a concentration range of 0.00 to 0.80 mg/L, with a correlation coefficient exceeding 0.9998. Average spike recovery was found in a range of 88% to 91%, with a relative standard deviation (RSD) between 1.1% and 4.0%. The minimum detection limit of source is 0.013 mg/Kg. The method can be used for rapid test on formaldehyde preservative in dried bean milk cream.