Simultaneous Resolution of Clenbuterol Hydrochloride Enantiomers and Propranolol Hydrochloride Enantiomers using Thin-Layer Chromatography

摘要

Resolution of cienbuterol hydrochloride enantiomers and propranolol hydrochloride enantiomers was achieved by thin-layer chromatography using methyl-(3-cyclodextrin impregnated silica gel GF254 plates. The effects of stereoselective auxiliary (acetonitrile/different alcohols) were studied. Resolution of cienbuterol hydrochloride enantiomers could be realized by alcohols such as ethanol, n-butanol, 2-butanol or tert-butanol together with acetonitrile, and propranolol hydrochloride enantiomers could only be resoluted by ethanol or n-butanol together with acetonitrile. The optical conditions of simultaneous resolution were obtained as follows: 15.00g silica gel GF254 plates impregnated with 1.00g methyl-β-cyclodextrin, acetonitrile/n-butanol (V/V = 90:10) mixed solvent as spreading solvent, being resolved under ambient temperature. Under these conditions, R_f value of resolution of cienbuterol hydrochloride enantiomers is 3.47, R_f value of resolution of propranolol hydrochloride enantiomers is 3.35. The two couples of enantiomers were all achieved base line separation.