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Free Amino Acids Fingerprint Analysis for the Quality Evaluation of Oriental Latrine Fly Larvina by RP-HPLC Pre-column derivatization Method

机译:RP-HPLC预柱衍生法对东方厕所飞幼虫的质量评价的游离氨基酸指纹分析

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A simple and reliable method of high-performance liquid chromatography with pre-column derivatization was developed to evaluate the quality of Oriental Latrine Fly Larvina through establishing chromatographic fingerprint. The separation was performed on a welch Ultimate XB-C8 column with a liner gradient elution of acetonitrile and acetate buffer. The developed method was validated by precision, stability and recovery. This study indicated that the chromatographic fingerprint analysis can be readily utilized as a quality control method for oriental latrine fly larvina.
机译:开发了一种简单可靠的高性能液相色谱方法,具有预域衍生化,以评估东方紫红素飞幼虫的质量通过建立色谱指纹。在韦尔奇终极XB-C8柱上进行分离,亚乙腈和乙酸盐缓冲液的衬垫梯度洗脱。通过精度,稳定性和恢复验证开发方法。该研究表明,色谱指纹分析可以容易地用作东方厕所飞幼虫的质量控制方法。

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