Mutagenesis of catA from Pseudomonas sp. B3-1 to Enhance Catechol Accumulation

摘要

Pseudomonas sp. B3-1, a wild strain isolated from soil, produced catechol from benzoate and accumulated it outside the cell. catA, a gene encodes a catechol 1, 2-dioxygenase in the bioconversion of aromatic compounds, plays the central role in accumulation of catechol. Mutant of the catA gene is disrupted without blocking the transcription of downstream genes was analyzed. The result showed that the mutant had less catechol 1, 2-dioxygenase activity, only 1/3 of strain B3-1's. The mutant produced a maximal amount of catechol (1.22 mg/ml) from 4 mg/ml of sodium benzoate after growing for 48 h. The conversion rate of benzoate to catechol was 51.5% on a molar basis.