ER-a36 participate growth inhibiting which induced by Tamoxifen in gastric cancer cell line BGC823

摘要

We aimed to investigate the expression and location change of ER-a36 in BGC823 when dealt with TAM. Cell growth was examined by WST-1 and assayed by microplate reader. ER-a36 expressions in mRNA and protein were assayed by RT-PCR and Western-blot method and were quantitated. The location of ER-α36 in cell was examined by immunofluorenscence methods. The cell numbers significant decreased at all TAM treatment groups compared with control group and cell growth rate had a concentration-dependent of TAM. ER-α36 mRNA and protein expressions decreased at all TAM treatment groups compared with control group and had a concentration-dependent of TAM. ER-α36 is localized on the membrane and cytoplasm of BGC823 cell, and its location did not change when dealt with TAM TAM could inhibit the growth of BGC823 cell, and ER-α36 may transmit growth regulated signal of TAM through a membrane-based pathway in gastric cancer.