Fast Automated Synthesis of An Antimicrobial Peptide Analog Using UV-Monitoring on the Tribute

摘要

@@ The IntelliSynth UV-Monitoring and Feedback Control System available on the Tribute~R Peptide Synthesizer from Protein Technologies, Inc. Is an invaluable tool for peptide synthesis. Unlike other UV-monitoring systems available on the market today, the IntelliSynth system is the only on-line system that can monitor the deprotection reaction in real-time.Other UV-monitoring systems use an external monitor that can only take one measurement after a 10 minute deprotection step. They require the nitrogen and NMP flow rates to be calibrated prior to every synthesis, and require extra methanol rinses between UV-measurements to remove bubbles and clear the lines. They cannot control the length of the deprotection reactions.