Solid Phase Peptide Synthesis of Antimicrobial Peptides for cell Binding Studies: Characterization Using Mass Spectrometry

摘要

The ultimate goal of the project is to synthesize three specific peptides (cercropin Pl, cercropin A and PGQ) in order to determine their anti- microbial activity. To expedite this goal, mass spectrometry was used to identify products of the solid phase synthesis and thereby optimize synthetic conditions. Three different analytical approaches - MALDI-TOF, LC-electrospray ionization (ESI), ion trap MS and direct infusion ESI Ion Trap MS - were examined. Of particular consideration was the selection of methodology compatible with instrumentation capabilities at Soldier Systems Laboratory, Natick, MA for eventual incorporation of the techniques in-house. Direct infusion ESI Ion Trap MS provided the best results and most realistic analytical approach for purposes of this program given the instrumentation available.