Partition of amyloglucosidase in poly (ethylene glycol) / sodium-poly (acrylate) aqueous two-phase systems

摘要

Amylolitic enzymes have been widely investigated in order to improve industrial process of starch hydrolysis. Liquid-liquid extraction by aqueous two-phase systems (ATPS) has proved to be an important tool for separating and purifying mixtures of biomolecules by extraction. In this work a face centered design combined with the response surface technique was employed to evaluate the partition of amyloglucosidase (AMG) in ATPS composed by poly ethylene glycol (PEG) and sodium poly acrylate (NaPA) as a function of pH, PEG and NaPA concentration. The experimental results were submitted to analysis of variance (ANOVA) and showed that partition of AMG was influenced significantly (p<0.05) by pH and NaPA concentration (% (w/w)). High partition coefficient values (2.018) was found in ATPS composed by PEG 4000 (11.5% (w/w)) with sodium poly acrylate (11.4% mass), at pH 6.5, resulting in high values of enzyme activity in the PEG rich phase.