Experimental Study on Detection Method of Micro-fluidic chip

摘要

A fluorescent markers based two-dimensional parallel detection technique of Micro-fluidic chip is studied. In a special designed fluid field of test sample, excite fluorescence of micro-spheres probe by pulse-laser and employ high sensitivity CCD to catch it. The entire information from micro-fluidic chip can be obtained via a computer post-processing of the caught signal of fluorescence. Analyzed the probe flow state and fluorescence signal intensity of detection program. Established the expressions of fluorescence signal intensity and the parameters of detection system. Validation experiments indicate that this detection method has the advantages of rapid, accurate and high sensitivity.