Preparation of Chitosan/TPP Nanoparticles used as Carrier in Enzyme Immobilization

摘要

Objective This work investigated the preparation of chitosan nanoparticles used as carriers for immobilized enzyme. Methods The nanoparticles were prepared by ionotropic gelation method. Response surface methodology was used in the preparation of nanoparticles. The morphologic characterization of chitosan nanoparticles was evaluated by scanning electron microscope and atomic force microscope. Results Using an appropriate experimental design, we have studied the significant influence factors (pH of the solution, concentrations of the chitosan, ratios of components) in the preparation of chitosan/triphosphate (TPP) nanoparticles.