Screening of acrylamide bioconversion bacterium and of its nitrile hydratase producing conditions

摘要

A strain T3, which has the better ability of acrylamide bioconversion, was isolated from activated sludge in wastewater treatment system at an acrylic fiber factory. T3 is primarily identified as Rhodococcus sp. according to its mor-phological observation. The process conditions producing nitrile hydratase for T3 were investigated. The results indicated that optimal conditions are initial mediuM pH 7.5, culture temperature 35 °C and culture time 72h; And the study also showed that 0.05g/L Co2 + is very important to improving enzyme activity. Under the optimum fermentation conditions, the enzyme activity of T3 cell culture fluid may reaches its maximum value 128. 3U/mg, which increased by 55.1% than before.