Bioactive Compounds in Moringa oleifera: Isolation, Structure Elucidation, and Their Antiproliferative Properties

摘要

A systematic study for the isolation and structure elucidation using thin-layer chromatography (TLC)/electrospray ionization mass spectrometry (ESI-MS) of microwaved methanolic extract of different sections of the tropical fruit Moringa oleifera is described. Different tissues of Moringa oleifera such as seed coat (inner skin skeleton), pulp and seeds when subjected to electrospray ionization mass spectrometry provided 4-(±-L-rhamnopyranosyloxy) benzyl glucosinolate and its corresponding three mono acetyl isomers. Benzyl glucosinolate at m/z 408, its parahydroxy derivative and its higher homologue are also profiled. Biological active myrosinase hydrolyzed products, 1-iso thiocyanate-(5-methylsulfinylpentane), and 1-isothiocyanato-3-methylsulfonyl-propane presumably by the hydrolysis catalyzed by myrosinase were also characterized. The identification of O-ethyl-4-[α-L-rhamnosyloxy benzyl]-carbamate from M. oleifera pulp provided m/z 341 is also described. Collision-induced dissociation mass spectra of anions present in Moringa oleifera exhibited product ion at m/z 97, characteristic of sulfate moiety (HSO_4~-) in the molecular structure of glucosinolates. The proliferation inhibitory effects of Moringa oleifera seed, seed coat, pulp (inner skin skeleton) and skin on human colon cancer HT-29 cells and Caco-2 cells are investigated in these studies.