Preparation and Characterization of Nanostructured Magnetic PMA Microspheres Immobilized with HCV Antibody

摘要

A novel method for preparation of monodisperse and strong magnetic poly methyl acrylate (PMA) microspheres immobilized with Hepatitis C Virus (HCV) antibody for use in Magnetic Immunoassay was investigated. Fe3O4 nanoparticles were obtained by chemical coprecipitate method. The PMA seed particles were synthesized by miniemulsion polymerization, and the nanostructured magnetic PMA microspheres were prepared by monomer swelling polymerization, in which the non-magnetic PMA seed particles were swollen by the methyl acrylate-based magnetic fluid. The amino groups on the surface of magnetic PMA microspheres were obtained by ethylenediamine ammonolysis reaction, and then HCV antibody were covalently immobilized on the surface of magnetic PMA microspheres by glutaraldehyde. The morphology, size distribution and magnetic properties of magnetic PMActron Microscopy (SEM) and Vibrating Sample Magnetometer (VSM), respectively. The results showed that the magnetic PMA microspheres had an average size of 5.416μm and the saturation magnetization was found to be 5.839emu/g. The capacity of amino groups and HCV antibody on the surface of magnetic PMA microspheres were measured by atomic adsorption spectrophotometer (AAS) and UV-Vis spectrophotometer (UV-Vis), respectively. The results showed that the capacity of the amino groups was 0.659mmol/g and the HCV antibody had high immobilization efficiency.