Effect of pH and inoculum percentage on canthaxanthin production by Dietzia maris

摘要

Dietzia maris was isolated from our laboratory and was grown in Yeast Extract-Peptone-Glucose medium at 37 °C and 180 rpm. The pH of the medium was varied between 1 and 14 and inoculum percentage between 0.5 % and 10 %. Considerable growth and pigmentation was observed at pH between 5 and 12 and at all inoculum percentage, with the maximum yield at pH 5.5 and 2 % inoculum. The pigment was extracted by solvent extraction method using methanol. The pigment extracts were analyzed by scanning the absorbance with a UV - Vis spectrophotometer and the maximum peak was obtained at 483 nm. The pigment was identified as canthaxanthin by HPLC analysis.