PRODUCTION OF LYSIN-FREE PHAGE INOCULUM AND PRODUCTION OF LYSIN

摘要

A method for producing a lysin-free phage inoculum, which comprises: a) inoculating a growing Streptococcal culture with phage; b) incubating the culture for plurality of lytic cycles of phage until the cells are completely lysed to obtain a lysate; and c) removing cell debris and free lysin from the lysate to form a lysin-free phage suitable for use as an inoculum. Also disclosed is a method of producing lysin.