Proteomic Characterization of Human Plasma Protein Adsorption onto Biomimetic Glycocalyx Surfaces

摘要

Protein adsorption is fundamental to understand thrombosis and to design biocompatible materials for medical implants, devices, biosensors and drug delivery. We report a proteomic approach to characterize multiple human plasma proteins adsorbed onto four different types of model surfaces (silicon oxide, dextranized silicon, polyester-based polyurethane, and dextranized polyurethane) using two-dimensional electrophoresis and mass spectroscopy. The surfaces of silicon wafers and polyurethane were chemically modified with covalent attachment of dextran to the substrate to mimic the endothelial cell glycocalyx surface of a blood vessel lumen. Surface properties such as topography and hydrophobicity/hydrophilicity were determined and analyzed using atomic force microscopy and contact angle measurement. We show that dextranization of both silicon and polyurethane surfaces inhibits protein adsorption. These results suggest that dextran coatings for blood-contacting biomedical applications provide a promising approach for reducing inflammatory and thrombotic responses.