EFFECTS OF PULSE VOLTAGE STIMULATION ON FRUIT BODY FORMATION IN LENTINULA EDODES CULTIVATION

摘要

Pulsed high voltage was applied to natural logs for cultivation of mushroom to clarify an effect of the pulse voltage stimulation on fruit body formation. Inductive energy storage system was employed to construct a pulsed power generator to generate narrow pulse over 100 kV with compact size. Copper thin fuse was used as opening switch to interrupt large circuit current in short time. Four stages Marx generator was used to supply a large current to a secondary energy storage inductor. The output voltage of the inductive energy storage system pulsed power generator was 120 kV with 50 ns pulse width at 5 kV charging voltage to the primary energy storage capacitor. The output voltage of 50 ns pulse width was applied to the natural logs of Lentinula edodes (Shiitake mushroom) cultivation as the stimulation for the fruit body formation. The total weight cropped by fifteen logs was 2.29 kg at fifty times 50 kV pulse voltage stimulations and was larger than 1.09 kg at one 50 kV pulse stimulation case. The deviation of the mushroom yield among the logs decreased with the pulse voltage stimulation. The hydrophobic protein, which was predicted to contribute to the fruit body formations, was confirmed to be released from the vegetative hyphae by applying the pulse voltage.