Separation and Determination of Glycyrrhizic acid and Liquiritin in Licorice using SPE-RP-HPLC

摘要

Glycyrrhizic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our stud, different methods were compared to separate GA and liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine Glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used. The regression line for GA was y=15340.6x+12.81, the regression for liquiritin line was y=9269.1x+20.63, The recovery rates were 101.3% and 99.4%; RSD were 1.01% and 0.92%.