Modulating DNA Adsorption On Silica Beads in An Electrically Actuated Microfluidic Device

摘要

DNA adsorption/desorption on solid surfaces serves as the underlying mechanism for the vast majority of DNA preconcentration and separation techniques [1-7] that are available for genetic analysis of biological samples [8-10]. Silica surfaces are the most common ones that are applied for DNA extraction and concentration [1-3, 5, 11]. DNA interaction with silica surface has been intensively studied to illustrate mechanistic details of these adsorption/desorption processes [11-18]. In the majority of reported protocols, DNA extraction from biological samples was performed by inducing DNA binding onto silica surface in a high ionic strength buffer in the presence of high concentration salts (e.g. 6M Guanidium HCl) and later eluting DNA out in a low ionic strength buffer [1-3, 5, 11, 16, 17].