Creating a detailed map of the wheat flour proteome: A critical step in understanding the effects of environment on flour quality and immunogenic potential

摘要

Proteomic strategies combining high-resolution 2-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (MS/MS) offer considerable potential for understanding the effects of environment on flour quality and immunogenic potential. However,it is challenging to create a detailed map of the wheat flour proteome, largely because of the complex nature of the major gluten proteins. Using improved methods, a comprehensive proteomic map of wheat flour from the bread wheat Butte 86 was developedin which 230 proteins were associated with specific gene sequences. The proteomic map was used to identify proteins that are differentially expressed in developing Butte 86 grain in response to post-anthesis fertilizer. These results coupled with recentsuccess in the genetic transformation of Butte 86 facilitate targeted efforts to alter flour composition in the US bread wheat and to more clearly define the roles of individual proteins in flour quality and in the response of the grain to the growth environment.